otan tästä sitaatin. katsahdin artikkelia etsiessäni karttaa diHETE molekyyleist ja löysin kartn C22:4 omega molekyyleistä ja luulin ensin virheeksi koska se oli yhtä laaja kuin C-20:4 omega 6 kartta. Muta niin se vain on että se on laaja kartta. Tämä löytöhappo voi betaoksidoitua kyllä takaisin arakidonihapoksi, jonka elongoituma se on. Onhan se ollut tietty n-6 kartassani jo aikaa, mutta sen merkitys ei ole ollut tiedossani.
Adrenic Acid Metabolites as Endogenous Endothelium-Derived and Zona Glomerulosa-Derived Hyperpolarizing Factors
Abstract
Adrenic acid (docosatetraenoic acid), an abundant
fatty acid in the adrenal gland, is identical to arachidonic acid except
for 2 additional carbons on the carboxyl end. Adrenic acid is
metabolized by cyclooxygenases (COXs), cytochrome P450s, and lipoxygenases (LOXs);
however, little is known regarding the role of adrenic acid and its
metabolites in vascular tone. Because of its abundance in the adrenal
gland, we investigated the role of adrenic acid in vascular tone of
bovine adrenal cortical arteries and its metabolism by bovine adrenal
zona glomerulosa cells. In adrenal cortical arteries, adrenic acid
caused concentration-dependent relaxations, which were inhibited by the
epoxyeicosatrienoic acid (EET-)antagonist 14,15-epoxyeicosa-5(Z)-enoic acid
and the cytochrome P450 inhibitor SKF-525A. The large-conductance
calcium-activated potassium channel blocker iberiotoxin or removal of
the endothelium abolished these relaxations. Reverse-phase high-pressure
liquid chromatography and liquid chromatography/mass spectrometry
isolated and identified numerous adrenic acid metabolites from zona
glomerulosa cells, including dihomo-epoxyeicosatrienoic acids and
dihomo-prostaglandins. In denuded adrenal cortical arteries, adrenic
acid caused concentration-dependent relaxations in the presence of zona
glomerulosa cells but not in their absence. These relaxations were
inhibited by SKF-525A, 14,15-epoxyeicosa-5(Z)-enoic acid, and
iberiotoxin. Dihomo-16,17-epoxyeicosatrienoic acid caused
concentration-dependent relaxations of adrenal cortical arteries, which
were inhibited by 14,15-epoxyeicosa-5(Z)-enoic acid and high potassium.
Our results suggest that adrenic acid relaxations of bovine adrenal
cortical arteries are mediated by endothelial and zona glomerulosa cell
cytochrome P450 metabolites. Thus, adrenic acid metabolites could
function as endogenous endothelium-derived and zona glomerulosa-derived
hyperpolarizing factors in the adrenal cortex and contribute to the
regulation of adrenal blood flow.
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